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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 45,
  • Issue 5,
  • pp. 743-746
  • (1991)

Laser-Induced Fluorescence Detection of Rhodamine-6G at 6 × 10−15 M

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Abstract

We have demonstrated sensitive fluorescence detection of rhodamine-6G in aqueous solutions down to ∼10<sup>−15</sup> M with a detection limit of 6 × 10<sup>−15</sup> M. The working curve was observed to be linear over four orders of magnitude and could be extended to higher concentrations. Single molecules of rhodamine-6G had a transit time of 1.8 ms through the 11 pL, probe volume. At 2 × 10<sup>−14</sup> M, the probability of a molecule being in the 11 pL probe volume is ∼0.1. Analysis of the data indicates that the signal-to-noise ratio for the detection of a single molecule of rhodamine-6G is ∼1.

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