Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 52,
  • Issue 3,
  • pp. 348-355
  • (1998)

Confocal Direct Imaging Raman Microscope: Design and Applications in Biology

Not Accessible

Your library or personal account may give you access

Abstract

A confocal direct imaging Raman microscope (CDIRM) based on two synchronized scanning mirrors, a monochromator, and two charge-coupled device (CCD) cameras has been developed. With this system it is possible to make both Raman spectra of a small measurement volume and images of a larger sample area in one specific Raman band. The spatial resolution of the system was determined for two limiting situations: a small sphere and a thin layer. The image of a 0.282 mu m sphere appeared to have a full width at half-maximum (FWHM) of 1.2 mu m in the axial and 0.37 mu m in the lateral direction, whereas the image of a 275 nm layer showed an FWHM of 1.4 mu m in the axial direction. Confocal Raman images were made of the DNA and protein distribution in polytene chromosomes with a relatively weak Raman signal [0.1 photons/(second.pixel)]. Further, a three-dimensional Raman image of the drug distribution in a phthalocyanine-incubated fixed cell is presented. These examples show that the CDIRM can be used to image samples with a weak Raman signal and that three-dimensional images of the distribution of specific molecules in a sample can be made.

PDF Article
More Like This
Design and application of a confocal microscope for spectrally resolved anisotropy imaging

Alessandro Esposito, Arjen N. Bader, Simon C. Schlachter, Dave J. van den Heuvel, Gabriele S. Kaminski Schierle, Ashok R. Venkitaraman, Clemens F. Kaminski, and Hans C. Gerritsen
Opt. Express 19(3) 2546-2555 (2011)

Chemical imaging with a confocal scanning Fourier-transform–Raman microscope

Colin J. H. Brenan and Ian W. Hunter
Appl. Opt. 33(31) 7520-7528 (1994)

A handheld laser scanning confocal reflectance imaging–confocal Raman microspectroscopy system

Chetan A. Patil, Christopher L. Arrasmith, Mark A. Mackanos, David L. Dickensheets, and Anita Mahadevan-Jansen
Biomed. Opt. Express 3(3) 488-502 (2012)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved