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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 67,
  • Issue 8,
  • pp. 873-883
  • (2013)

High-Throughput, High-Resolution Echelle Deep-UV Raman Spectrometer

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Abstract

We constructed an ultrahigh-throughput, high-resolution ultraviolet (UV) Raman spectrograph that utilizes a high-efficiency filter-stage monochromator and a high-dispersion Echelle spectrograph. The spectrograph utilizes a total of six mirrors and two gratings, with an overall efficiency at 229 nm of ?18%. The limiting resolution of our spectrometer is 0.6 cm<sup>?1</sup> full width half-maximum (FWHM), as measured for 229 nm Rayleigh scattering. Use of a 1 mm-wide entrance slit gives rise to an approximately 10 cm<sup>?1</sup> FWHM resolution at 229 nm. The ultrahigh spectrograph throughput enables ultrahigh signal-to-noise ratio, deep UV Raman spectra that allow us to monitor <1% changes in peptide bond composition. The throughput is measured to be 35-fold greater than conventional deep UV Raman spectrometers.

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